FIGURE 7.
PfPAT mediates entry of fenpropimorph into yeast cells. BY4741-derived end3Δ strain lacking the END3 gene harboring the pBEVY-U vector (open circles), end3Δfen2Δ pBEVY-U (open triangles), and end3Δfen2Δ-pPFPATf (closed triangles) were precultured in SV medium supplemented with 100 μm of pantothenate, washed twice, and inoculated to an initial cell density of 2 105 cells/ml in YPD medium in the absence (A and C) or presence (B and D) of 1 μg/ml fenpropimorph. E and F, for agar assay, cells were cultured in SV medium supplemented with 100 μm of pantothenate and washed twice and 10-fold serial dilutions of cells were plated onto YPD medium (E) or YPD medium supplemented with 1 μg/ml fenpropimorph (F) and incubated at 30 °C.