FIGURE 2.

AICAR inhibits H₂O₂-induced phosphorylation of caveolin-1 via activation of AMPK. A, cells were treated with each concentration of AICAR for 2 h. B, cells were treated with each concentration of DPY for 1 h and then stimulated with 2 mm AICAR for 2 h. C, cells were treated with 8 μm DPY for 1 h and then stimulated with 2 mm AICAR for 2 h followed by H₂O₂ (2 mm) stimulation for 30 min. D, densitometry of p-caveolin-1 in C is shown. E, densitometry of p-c-Abl in C is shown. F, cells were treated with each concentration of IODO for 1 h and then stimulated with 2 mm AICAR for 2 h. G, cells were treated with 0.4 μm IODO for 1 h and then stimulated with 2 mm AICAR for 2 h followed by H₂O₂ (2 mm) stimulation for 30 min. H, densitometry of p-caveolin-1 in G is shown. I, densitometry of p-c-Abl in G is shown. A[en]E, representative blots are shown. *, p < 0.01.