FIGURE 4.

AMPK mediates AICAR effects on c-Abl and caveolin1 phosphorylation. A, cells were co-transfected with both AMPKα1 and -α2 siRNAs (two independent oligos) (α1, (PRKAA1)-CCAUACCCUUGAUGAAUUA; α2 (PRKAA2)-CGACUAAGCCCAAAUCUUU). Three days after transfection cells were stimulated with 2 mm AICAR for 2 h followed by H₂O₂ (2 mm) stimulation for 30 min. The amounts of p-c-Abl and p-caveolin-1 were examined by Western blotting. B, densitometry of p-c-Abl in A is shown. C, densitometry of p-caveolin-1 in A is shown. D, cells were co-transfected with both AMPKα1 and -α2 siRNAs (two independent oligos: α1′, (PRKAA1)-GCCCAGAGGUAGAUAUAUG, α2′ (PRKAA2)-GAGCAUGUACCUACGUUAU). Three days after transfection, cells were stimulated with 2 mm AICAR for 2 h followed by H₂O₂ (2 mm) stimulation for 30 min. The amounts of p-c-Abl and p-caveolin-1 were examined by Western blotting. E, densitometry of p-c-Abl in D is shown. F, densitometry of p-caveolin-1 in D is shown. A and D, representative blots are shown. *, p < 0.01; NS, not significant.