FIGURE 5.

Inhibitory effect of AMPK on caveolin-1 phosphorylation under oxidative stress is dependent on c-Abl. A, cells were treated with 10 or 20 μm imatinib mesylate for 24, 48, or 72 h before stimulation with H₂O₂ (2 mm) for 30 min. B, densitometry of p-caveolin-1 in A is shown. C, densitometry of p-c-Abl in A is shown. D, cells were transfected with siRNA against c-Abl. Three days after transfection cells were stimulated with 2 mm AICAR for 2 h followed by stimulation with H₂O₂ (2 mm) for 30 min. E, densitometry of p-caveolin-1 in B is shown. A and D, representative blots are shown. *, p < 0.01; NS, not significant.