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Light scattering analysis of varying concentrations of tobacco Rubisco activase. Different concentrations of tobacco Rubisco activase (18–142 μm) were loaded onto a Superdex 200 10/300 column and eluted at 0.5 ml·min−1. Refractive index (top panel) and right angle scattering were measured and used to calculate the molecular mass (lower panel) as described under “Experimental Procedures.”
