FIGURE 5.

Ab initio monomer-hexamer equilibrium of Rubisco activase generated using GASBORMX. a, fit of the model equilibrium to the SAXS data at 0.1 mg·ml⁻¹ sample concentration. b, volume fractions of monomer and hexamer calculated from the best weighted combinations of the model components at each concentration. The corresponding discrepancy in the fits of the models to the data at each concentration are shown above the bars. Error bars, Errors in the volume fractions shown are those propagated from the solution of linear equations used in OLIGOMER (46). S.D. c, ab initio monomer (red spheres) and hexamer (green spheres) reconstructed from the SAXS data at 2.4 μm (0.1 mg·ml⁻¹). The hexamer model is shown overlaid with the structure of tobacco Rubisco activase (Protein Data Bank ID code 3ZW6). d, rotation of the view in a by 90º in the horizontal.