TABLE 1.

Absorption maximum, opsin shift, and retinal configuration of the WT and of various HwBR mutants both in the dark and light

Opsin type	λmax	Δυ	All-trans
			Dark-adapted	Light-adapted
	nm	cm−1	%
WT (HwBR)	552		79 ± 2	90 ± 3
D123A	533	646	61 ± 2	60 ± 2
M126A	543	300	60 ± 4	75 ± 3
S149A	536	541	69 ± 2	76 ± 2
A223N	548	132	77 ± 2	81 ± 3
A223T	539	437	78 ± 2	96 ± 2
D123A/M126A	521	1078	65 ± 3	58 ± 1
D123A/S149A	526	895	62 ± 3	69 ± 2
D123A/A223T	535	576	66 ± 2	70 ± 2
M126A/S149A	514	1339	70 ± 2	71 ± 3
M126A/A223T	525	932	81 ± 2	73 ± 2
S149A/A223T	524	968	81 ± 2	77 ± 3
D123A/S149A/A223T	520	1115	53 ± 1	48 ± 2
D123A/M126A/A223T	521	1078	71 ± 2	56 ± 1
D123A/M126A/S149A	506	1647	65 ± 2	58 ± 1
M126A/S149A/A223T	498	1964	73 ± 2	66 ± 2
D123A/M126A/S149A/A223T	503	1765	76 ± 1	72 ± 2