FIGURE 2.

FAB1 is required for transcriptional induction of ICL1, FBP1, PCK1, and IDP2. A, mRNA levels of the genes in gluconeogenesis and glycolysis were quantified by RT-qPCR. The mRNA level of each gene in WT cells in exponential growth phase in YPD was set as 1.0. The relative mRNA levels of the gene in different conditions are presented. YPD (stationary), cultures at 48 h in YPD. The y axis is plotted on a log scale. Three independent experiments were performed and analyzed by standard statistical analysis. Error bars, S.D. ***, the two values of interest have a statistically significant difference with p values lower than 0.05. Values are shown only for the relative mRNA levels in WT and fab1Δ cells in late stationary phase in YPD. The relative mRNA levels in exponential phase are either 1.0 (WT) or close to 1.0 (fab1Δ), but for simplicity, their values are not shown. B, mRNA levels of FBP1, PCK1, ICL1, and IDP2 in WT and fab1Δ cells were quantified by RT-qPCR. mRNA levels of each gene were calculated relative to the mRNA level in WT cells in exponential phase in YPD, which was set as 1.0. Two non-glucose carbon media, ethanol (n = 3) and fructose (n = 1), were used to induce the gluconeogenesis genes. Error bars, S.D. ***, the two values of interest have a statistically significant difference with p values lower than 0.05. Values are shown only for the relative mRNA levels in WT and fab1Δ cells in YPEtOH or YPFructose. The relative mRNA levels in exponential phase in YPD are either 1.0 (WT) or close to 1.0 (fab1Δ or cti6Δ), but for simplicity, their values are not shown.