FIGURE 4.

Transcription of Cat8 and Sip4, two key transcriptional activators of gluconeogenesis genes, is under control of glucose repression. A, RT-qPCR analyses showed that the mRNA level of SIP4, but not CAT8, was higher in tup1Δ cells compared with the level in WT cells (set as 1.0) in the exponential growth phase in YPD (n = 3). Error bars, S.D. ***, the mRNA levels of SIP4 have a statistically significant difference with p values lower than 0.05 between WT and tup1Δ cells. B, relative mRNA levels of CAT8 and SIP4 were analyzed by RT-qPCR in WT, fab1Δ, and cti6Δ cells either in late stationary phase at 48 h in YPD (left) or in YPEtOH for 15.5 h after shifting from YPD to YPEtOH (right) compared with the levels in WT cells (set as 1.0) in the exponential phase in YPD (n = 3). Error bars, S.D. CAT8 was highly induced in WT, fab1Δ, and cti6Δ cells in late stationary growth phase in YPD and in ethanol medium. Transcriptional induction of SIP4 was defective in fab1Δ and cti6Δ cells. Values are shown only for the relative mRNA levels in WT and fab1Δ cells in YPEtOH or YPFructose. The relative mRNA levels in exponential phase in YPD are either 1.0 (WT) or close to 1.0 (fab1Δ or cti6Δ), but for simplicity, their values are not shown.