FIGURE 12.

Disruption of N-P domain electric field by mutations. The N and P domains in E1Ca₂·AlF₄⁻·ADP are shown with the electric field lines for the wild type (WT) and all the nine mutants as indicated, otherwise as shown in Fig. 11. The enlarged area of the ATPase molecule is indicated with a schematic model in green dashed lines. The red arrow in the wild type (WT) shows the approximate movement of Glu³⁹⁴/Asp³⁹⁹ toward Lys⁶⁰⁵ during the EP transition (E1Ca₂·AlF₄⁻·ADP → E2·BeF₃⁻).