FIGURE 5.
Apo mlLRH-1 is more active than WT LRH-1 by enhancing stability in the alternate activation region. Binding affinities for various peptides derived from coactivator and corepressor proteins were expressed as Kd ± S.E. A, human and mlLRH-1 LBD bound to DLPC. B, apo human and mlLRH-1 (ml) LBD. C, differential HDX-MS between apo hLRH-1 and apo ml LRH-1 (ml) LBD mapped onto PDB 1YUC. The difference in percentage of deuterium incorporation is indicated by the colored scale bar. Figures were generated in PyMOL.