FIG. 5.
κB-Ras 1 protects IκBβ degradation. (A) HeLa cells cotransfected with hemagglutinin (HA)-tagged IκBβ and κB-Ras1 or empty vector were grown for 2 days followed by treatment with TNF-α for the indicated period of time. Similar experiments were done with three different IκBβ mutants: HA-IκBβΔ152-192 (B), HA-IκBα(1-65)/IκBβ(50-359) (C), and HA-IκBα (1-65)/IκBβ(50-359)Δ(152-192). ns, nonspecific binding.