FIG. 3.

GR-dependent NF1 binding is reversible. (A) Experimental design. (B) Autoradiogram of DMS in vivo footprinting developed by primer extension. Lanes: A, no hormone; B1 and B2, corticosterone agonist at time 13 h (B1) and 23 h (B2); C, oocytes exposed to the indicated antagonist at time 13 h and analyzed as double samples. Grey arrows, right, protected bands; black arrowheads, reference bands.Scans are averages of each double lane, with indicated binding sites below. (C) Quantified DMS methylation of the two guanosines in the NF1 site, with double samples for the indicated lanes. (Right) The level of NF1 binding without hormone (bars A) is used as reference, i.e., 100% (double arrow).