FIG. 4.

NF1-DNA binding depends on ligand-induced GR-GRE binding also in the presence of the antagonist RU486. MMTV reporter DNA- and GR-NF1 mRNA-injected oocytes were treated with the indicated ligands for 10 h. Two pools of 10 oocytes each were analyzed for transcription. (A) Diagram displaying the amount of mRNA expressed as a percentage of the agonist response. (B) DMS in vivo footprinting to quantify GR-GRE binding, an average of GRE II to IV (top), and NF1-DNA binding (bottom) from two pools of five oocytes each. (C) SacI in situ access analysis, showing the increase in cutting in relation to the mock-treated control. SacI bands (S) and HinfI bands (H) are indicated on the left. Error bars signify the two values of the double samples.