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. 2004 Apr;24(7):3036–3047. doi: 10.1128/MCB.24.7.3036-3047.2004

FIG. 7.

FIG. 7.

NF1 stimulates MMTV transcription in the presence of Oct1. Oocytes were injected with 2 ng of pMMTV:M13 mixed with 0.4 ng of AdML reporter construct 18 h before hormone treatment and then with 3.8 ng of GR and/or Oct1 mRNA and/or 0.4 ng of NF1 mRNA as indicated 14 h before hormone treatment. Double samples without hormone and triple samples with hormone, 10 oocytes in each pool, were taken for RNA and DNA analysis. Hormone-treated oocytes containing GR only were used as reference (100%). Error bars signify the 95% confidence interval. Student's t test of the hormone-treated oocytes containing GR and Oct1 in the absence (258% ± 5% [mean ± standard deviation]; n = 3) and presence (325% ± 3%; n = 3) of NF1 rendered a P value of 0.105. The AdML RNA was not used as reference due to hormone- and protein context-dependent systematic variation.