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. 2013 Jun 7;11:e0163. doi: 10.1199/tab.0163

Figure 2.

Figure 2.

Meristematic activity in early leaf primordia of Arabidopsis. Development of primordia of eighth foliage leaves (Leaf 8 primordia) monitored with the pCYCB1;1::GUS-DB reporter gene, which acts as a specific marker for the G2/M phase of the cell cycle. Blue color indicates the expression of the reporter gene. Arrows indicate cells at the margins of leaf primordia. (A and B) Scanning electron micrographs of gl1 leaves. Bar, 50 µm. (A) Leaf 8 primordium (indicated by 8) 50 µm in length. Also shown are Leaf 7 (7) and stipules (S) of older leaves. (B) Leaf 8, 0.4 mm long. Arrow indicates enlarged cells at margin. (C) Cross section of Leaf 8 primordium at stage of Leaf 8 primordium (indicated by 8) 50 µm in length. (D) Cross section of Leaf 8 primordium 0.16 mm in length, sectioned at a distance 25% of total length above the base. Bar, 50 µm. (E) Cleared Leaf 8 1.2 mm in length. Bar, 0.5 mm. (F and G) Cross sections of Leaf 8 primordia 1.5 mm in length. Bar, 50 µm. (F) Sections cut at a distance 25% of total length above the leaf base. (G) Section cut at a distance 50% of total length above the leaf base. Modified from Donnelley et al. (1999; Dev. Biol. 215: 407–419 with permission from the authors.