Patterning of marginal serration.(A,B), Wild-type leaf primordia (A) compared to a cuc2–3 mutant with a smooth leaf margin (B).(C), Overlay image of (A) and (B) showing that the cuc2–3 mutant has a defect in serrated margin outgrowth.(D–F) Early development of serration. D, Propidium-iodide stained cell image; (E) auxin maxima visualized by DR5rev::GFP signal; (E), an overlay of (D) and (E). Arrows indicate positions where teeth are predicted to develop.(G–I), Auxin maxima (arrowheads) along the leaf margin seen in the wild-type (G), cuc2–3 mutant (H), and pin1–8 mutant (I) leaf primordia expressing DR5rev::GFP. Note that the cuc2–3 mutant failed to maintain distinct auxin maxima; pin1–8 has irregularly dense but smaller auxin maxima. Modified from Kawamura et al. (2010).