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. 2013 Jan 30;33(5):1927–1939. doi: 10.1523/JNEUROSCI.2080-12.2013

Figure 6.

Figure 6.

Immunohistochemistry and quantitative analysis of expression of Olig2 and GST-Pi in brain sections from 5-week-old Klotho+/+ and Klotho−/− mice. A–D, IHC images show examples of Olig2 nuclear staining patterns at 4× (A, B) and 20× (C, D). Scale bars: A, B, 200 μm; C, D, 50 μm. A, C, KL+/+. B, D, KL−/−. The fimbria region is outlined. Hb, Habenula. E, Cell counting of Olig2+ was performed as described in Materials and Methods. The number of Olig2+ cells in fimbria were counted and the percentage of Olig2+ cells in KL+/+ and KL−/− graphed. *Statistical significance (p < 0.01, t test). Error bars indicate SD. F–I, IHC of brain sections with antibodies to the mature oligodendrocyte marker GST-Pi at 4× (F, G) and 20× (H, I). Scale bars: F, G, 200 μm; H, I, 50 μm. J, Quantitation of GST-Pi+ cells as described in Materials and Methods. The GST-Pi+ cells in fimbria were counted and the percentage of GST-Pi+ cells in KL+/+ and KL−/− graphed. *Statistical significance (p < 0.05, t test). Error bars indicate SD.