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Transcription factors involved in Klotho regulation of OPCs. A, Western blot analysis of STAT3 phosphorylation upon Klotho treatment of OPC cells. B, Luciferase assay of luciferase reporters in OPC cells. OPCs were transfected with the reporter plasmids with Renilla luciferase and treated with or without Klotho for 24 h followed by luciferase assay. The fold change comparing Klotho treated to control is shown. p values indicate statistical significance by t test.
