Table 1.
Production methods | 3plasmids | VV |
---|---|---|
Genome titer (qPCR, GC) | 2.3 × 1012 | 2.1 × 1012 |
Genomic titer (Southern blot, GC) | 5.6 × 1012 | 3.5 × 1012 |
Capsid titer (Silver staining, pts) | 5.0 × 1012 | 2.5 × 1012 |
Transduction titer (flow cytometry, TU) | 2.5 × 109 | 2.3 × 109 |
GC (Southern)/TU ratio | 2.2 × 103 | 1.5 × 103 |
Ad helper DNA contamination (measured by qPCR) | 0.034% | 0.024% |
AAV helper DNA contamination (measured by qPCR) | 0.11% | 0.0046% |
AAV-CMV-EGFP vector was produced in HEK293 cells by triple plasmid transfection (3plasmids) or was produced in HeLa S3 cells infected with Ad-AAV hybrid virus and vaccinia helper viruses (VV). TU, transducing unit; GC, genome copy; Pts, particles.