Table 1.

Characterization of AAV vector produced by triple plasmid transfection and vaccinia carrier

Production methods	3plasmids	VV
Genome titer (qPCR, GC)	2.3 × 1012	2.1 × 1012
Genomic titer (Southern blot, GC)	5.6 × 1012	3.5 × 1012
Capsid titer (Silver staining, pts)	5.0 × 1012	2.5 × 1012
Transduction titer (flow cytometry, TU)	2.5 × 109	2.3 × 109
GC (Southern)/TU ratio	2.2 × 103	1.5 × 103
Ad helper DNA contamination (measured by qPCR)	0.034%	0.024%
AAV helper DNA contamination (measured by qPCR)	0.11%	0.0046%

AAV-CMV-EGFP vector was produced in HEK293 cells by triple plasmid transfection (3plasmids) or was produced in HeLa S3 cells infected with Ad-AAV hybrid virus and vaccinia helper viruses (VV). TU, transducing unit; GC, genome copy; Pts, particles.