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. 2013 May 9;41(13):6739–6749. doi: 10.1093/nar/gkt354

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© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Nucleic acid binding of the HhH domain of FAAP24. (A) Representative EMSA with a 39 nucleotides ssDNA (ss39), a 30 bp dsDNA (ds30) and a 30 bp bubble DNA (B10) consisting of two 10 bp dsDNA stems separated by 10 unpaired nucleotides. This binding assay is performed in the presence of 0 (−), 0.2, 0.5, 1.7, 5, 16.7, 50 μM FAAP24 HhH domain protein. The complex is marked as ‘C’, the free DNA as ‘F’. (B) Fraction bound DNA is plotted as a function of the FAAP24 HhH domain protein concentration. Experiments were performed in triplicate using B10 (green), ds30 (blue) ss39 (red); for each individual binding experiment, a different symbol is used, and the line represents the calculated binding curve based on the quantified apparent dissociation constant based on all data points, R² of 0.9, 0.98, 0.92 for B10, ds30 and ss39 respectively.