Figure 5.

Theoretical curves for apparent IC₅₀ values for 1 (gray) and 2 (black) at [MBNL1] = 5 (dashed lines) and 50 nM (solid lines). The concentration of ligand required to observe half of MBNL1 in the free form, IC₅₀, depends on actual concentrations of MBNL1 and (CUG)₄. The curves were generated based on the experimentally determined affinities shown in Table 1.