Figure 6.
Native polyacrylamide gel for the RNA hairpins (rHP1 and rHP3) and triplexes. (A) rHP1 binds to modified RNA TFOs incorporated with LNA U residues. In lane 2, the control TFO (Table 1) does not bind to rHP1. In lane 6, rHP1 to 1-RUL1 molar ratio is 1:0.5. The fact that only two bands (rHP1 and triplexes) were observed suggests that the designed RNA triplexes form without alternative structures. (B) rHP1 binds to unmodified and modified RNA TFOs incorporated with 2-thio U residues. (C) The 2′-OMe modifications in TFOs destabilize RNA triplex formation. RNA triplex bands were not observed for TFOs incorporated with three or more 2′-OMe residues. (D) rHP3 binds to unmodified and modified TFOs incorporated with 2-thio U and LNA U residues.