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. Author manuscript; available in PMC: 2013 Jul 15.
Published in final edited form as: Methods Mol Biol. 2012;832:463–472. doi: 10.1007/978-1-61779-474-2_33

Fig. 1.

Fig. 1

Assaying UPS-GFP reporter levels in cells transfected with an mCherry-tagged protein by two-color flow cytometry. (a) Histogram displaying frequency distribution as a function of mCherry-fluorescence of cells transiently transfected with mCherry fluorescent protein (dashed line) and the mCherry-tagged protein under investigation (solid line). Populations of cells with low and high mCherry levels are indicated. (b ) Histograms displaying the UPS-GFP fluorescence intensities of cells in the low and high mCherry populations. The two mCherry populations can be compared by their mean GFP fluorescence intensities.