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. Author manuscript; available in PMC: 2013 Jul 15.
Published in final edited form as: Nat Immunol. 2012 Sep 23;13(11):1083–1091. doi: 10.1038/ni.2428

Figure 5. The B cell receptor (BCR) repertoire of GFPMYC positive GC B cells is enriched in high affinity variants.

Figure 5

(a) Diagrammatic representation of the 3 different populations purified from GC B cell pools of GFPMYC mice, 9 days after immunization with NP-KLH. (b) Distribution of GFPMYC+ and GFPMYC- subpopulations within these compartments. The detailed gating strategy to isolate the GC cell subsets is described in Supplementary Figure 1. (c) Doughnut charts show the fraction of sequenced Vh186.2 segments (CDR1 region) with a W33L mutation among all sequenced segments (W33L mutants/Total segments). See Supplementary Table 5 for additional details. The results correspond to the pooled data of two independent experiments, 2-3 mice analyzed per experiment. P value calculations are based on a Fisher's exact test.