Projections of AL to the SC. A, In situ image of callosal connections retrogradely labeled with the fluorescent tracer bisbenzimide (blue). BDA injection site (arrow) in the anterolateral border of the large acallosal zone lateral to V1. Blue staining at the injection site is attributable to tissue damage. White lines indicate the rostrocaudal level of the coronal sections shown in B and B′. B, Coronal section showing bisbenzimide-labeled callosal connections and injection site (arrow) in anterior part of acallosal zone. Right arrowhead marks V1/AL border, and left arrowhead indicates lateral border of acallosal zone. B′, Dark-field image of section adjacent to B, showing that BDA injection is confined to gray matter. C, Dark-field images of BDA-labeled patchy axonal branches terminating mainly in superficial layer (Op) and deep layer (InG). Scale bars: A, B, B′, 1 mm; C, 0.5 mm. For abbreviations, see Figure 1.