Figure 4.

BDNF immunoreactivity is significantly elevated in both the NAc core and shell on WD90 after cocaine self-administration training. A, Infusions during 10 d of saline or cocaine self-administration training (saline, n = 12; cocaine, n = 8). B, BDNF staining was significantly increased in the NAc core (***p < 0.0005, t₍₁₅₎ = 5.070, t test; saline, n = 11; cocaine, n = 6) and shell (*p < 0.05, t₍₁₈₎ = 2.537, t test; saline, n = 12; cocaine, n = 8) in the cocaine group on WD90 compared with saline controls. Data (mean ± SEM) are expressed as percentage of mean values in the saline group. C, Representative images of BDNF staining in the NAc on WD90 after saline (left) or cocaine self-administration (right). Top, Low-magnification images. Scale bar, 1000 μm. Middle and bottom, Higher-magnification images in core and shell, respectively. Scale bar, 100 μm.