Figure 3.

Adeno-associated virus (AAV) vector-mediated overexpression of GluA1 subunits in CeA. A, Validation of AAV plasmid carrying the cDNA encoding rat GluA1(flip) in cultured CHO cells. Control cells were transfected with AAV-GFP plasmid and samples from CeA tissues were shown as positive control. B,C, Western blot data (B) and summarized results (C) of GluA1, phosphorylated GluA1 at S845 and GluA2 proteins in CeA tissues collected from rats (n = 6/group) 7 or 14 d after bilateral intra-CeA infusion of 1 μl AAV-GluA1 virus or AAV-GFP virus. D, Representative micrographs of immunostaining and illustration after CeA microinjection, showing AAV-mediated eGFP expression within the defined boundary of CeA in low magnification (a, scale bar = 500 μm) and high magnification (b, scale bar = 50 μm), a diagram depicting successful injection sites (black dots) within the CeA (c), and CeA GluA1 expression from a rat in an uninjected side (d, scale bar = 50 μm) and in the other side injected with AAV-GluA1 in low (e, scale bar = 50 μm) and high magnification (f, scale bar = 20 μm). CeA preparations were prepared at post-injection day 10. **p < 0.01.