Figure 9.

Iba1+ microglia (green) target and phagocytose apoptotic cells, mitotic neural precursor cells, and neurons in the proliferative zones. A, Iba1+ cells (green) target cleaved caspase 3 (CC3)+ cells (red, white arrowhead). The DAPI+ nucleus (blue) of the Iba1+ cell is indicated with a white arrow. The vast majority of targeted neural precursor cells were CC3-negative. B, A Tbr2+ cell (red, arrowhead) expressing PCNA (blue) is targeted by an Iba1+ microglial cell (green). The microglial cell is also PCNA+ (blue, arrow). The majority of precursor cells targeted by microglia were PCNA+. C, Iba1+ cells (green) also target NeuN+ cells in the proliferative zones (red). Scale bar: (in A) A–C, 5 μm.