Fig. 2.

Parallel relationship between gastric mTOR signaling and expression of nesfatin-1/NUCB2 in fasting and obesity. A. Representative Western blots from fed, fasted or refed mice. Gastric nesfatin-1/NUCB2 and phospho-S6K (pS6) were blotted as described in the methods. β-actin was used as loading control. Quantification of image analysis of gastric nesfatin-1/NUCB2 and pS6 was expressed as mean±SEM. B. Results of quantitative PCR analysis of nesfatin-1/NUCB2 were expressed as fold increase from fed condition using GAPDH as loading control. Six stomachs were examined for each condition. *P<0.05 versus mice in the fed condition. C. Representative Western blots from lean (normal chow diet, NCD) or high fat diet (HFD) induced obese mice. Gastric nesfatin-1/NUCB2 and pS6 were examined. β-actin was used as loading control. Quantification of image analysis of gastric nesfatin-1/ NUCB2 and pS6 was expressed as mean±SEM. D. Results of quantitative PCR analysis of nesfatin-1/NUCB2 expression were expressed as fold increase from lean mice using GAPDH as loading control. Six stomachs were examined for each condition. *denotes P<0.05 versus lean mice.