Fig. 3.

Rapamycin regulates gastric mTOR signaling and nesfatin-1/NUCB2 expression. A. Representative Western blots from lean and HFD-induced obese C57BL/6J mice that received intraperitoneal injection of DMSO or rapamycin (1mg/kg). Nesfatin-1/NUCB2 and pS6 in gastric mucosa were detected using specific antibodies. β-actin was used as loading control. Quantification of image analysis of gastric nesfatin-1/NUCB2 and pS6 was expressed as mean ±SEM. B. Results of quantitative PCR analysis of nesfatin-1/NUCB2 were expressed as fold increase from control using GAPDH as loading control. Six stomachs were examined for each condition. *P<0.05 versus lean mice receiving DMSO.