Table 2.

The effects of NAC on curcumin/trolox-induced ROS levels.

	Untreated	Curcumin +Trolox	NAC	NAC+Curcumin +Trolox
ROS levels	100	185±11	107±6	135±13*

A2780 cells were treated with curcumin (10 μM) plus trolox (100 μM) for 2 hours in the presence or absence of 1 mM NAC.

ROS levels were examined by H₂DCFDA labeling with flow cytometry.

Cells without treatment but with H₂DCFDA dye were used as an untreated control.

Data (mean ± SD, n=3) were presented as percentages of the fluorescent level detected in untreated control cells.

^*P < 0.05 (column 4 versus column 2), using one-way ANOVA followed by Dunnett's analysis.