Fig. 7.

NALCN is required for the SubP-induced depolarization on the pacemaking activity in cultured ICCs. (A) RT-PCR detection of NALCN using tail clips within approximately 10 hours of birth. (B) Effects of SubP in pacemaking activity in wild-type and Nalcn knockout ICCs. Population data for the manipulations shown in left is expressed as the degree of depolarization. Values are mean ± SEM. (C) SubP-activated inward currents in small intestine single ICCs from wild-type and Nalcn mutant mice. Cells were held at −60 mV. Population data for the manipulations shown in left is expressed as the degree of currents. Values are mean ± SEM. (D) Effect of 2-APB on SubP-induced response in pacemaking activity.