Fig. 3.

Effect of Dex on the subcellular localization of rab-GDI isoforms in AR42J cells. AR42J cells were cultured in the absence (Control) or presence of Dex (50 nM) for 3 days. The cells were harvested and cytosol (C) and membrane (M) fractions were prepared and immunoblotted for rab-GDI isoforms using an antibody that recognizes both GDI-1 and GDI-2 as described in Methods. The asterisk indicates a non-specific band that is often observed between the two rab-GDI isoforms and most likely represents amylase.