Fig. 5.

Rab-GDI-1 and Hsp90 co-localize on AR42J cell membrane fractions. A. AR42J cells were treated with Dex for 2 days and harvested into lysing buffer. A post-nuclear supernatant (PNS) was prepared and applied to a 10-30% continuous iodixanol gradient as described in Methods. Fractions (150 μl) were collected from the top of the gradient and immunoblotted for rab-GDI-1 and Hsp90. B. Amylase levels in the fractions were determined and the data were normalized to 1, which represents the maximum level of amylase activity. C. Immunoblotting of gradient fractions for Rab3D, Rab5, Calnexin and syntaxin 6.