Fig. 6.
Geldanamycin inhibits CCK-8-induced amylase release. AR42J cells were plated in 6-well plates (3 × 105 cells/well) and treated with Dex (50 nM) for three days. The cells were washed with AAB and incubated in the presence or absence of the indicated concentration of geldanamycin for 30 min, washed once with AAB and incubated with no additions or CCK-8 (1 nM) for 40 min. Values for amylase release are expressed as percentage of the total cellular amylase present at the start of the 40 min incubation that was released into the medium. Results shown are means ± SEM from four experiments.