Figure 8.

NF-κB is required for BRG1 activation in myelinating cells. A, DRG/Schwann cell cocultures were stimulated for 6 days with ascorbic acid to induce myelination. Lysates were collected every two days. The NF-κB inhibitor SN50 (50 μg/ml) was added to some cultures for 48 h before cell lysis at the 6 day time point. Cell lysates were Western blotted for myelin basic protein (MBP) and tubulin (αTub). B, Lysates from myelinating DRG/Schwann cells were collected every 2 days. In some cultures, SN50 (50 μg/ml) was added for 48 h before cell lysis at 6 days. The cell lysates were immunoprecipitated with BRG1 antibodies, subjected to ATPase assays, and the amount of released Pi (inorganic phosphate) was quantified and normalized to the activity at day 0 before stimulating myelination by ascorbic acid addition. A one-way ANOVA used to calculate significance, followed by Tukey's post hoc test. The mean ± SEM is shown (n = 3; *p < 0.05).