Figure 3.

Increases in the CV and unitary failures with presynaptic Fmr1 deletion indicate quantal content is decreased. A, Left, middle, Example uEPSC1s from WT/WT and KO/WT pairs. Right, Same KO/WT traces scaled to match average WT/WT uEPSC1 and to highlight variability. Black traces are averages. B, Plot of CV versus genotypic combination of pre- and postsynaptic neurons. CV is higher with presynaptic deletion of Fmr1. C, Examples of unitary “failures” from a KO/WT pair. D, The proportion of unitary failures was higher in KO/WT pairs compared to WT/WT pairs. E, Scatter plot of CV versus uEPSC1 amplitude. F, Data from E merged into presynaptic KO versus presynaptic WT groups and binned. *p < 0.05; **p < 0.01. Individual uEPSC traces in A and C are a running average of three samples. Calibrations: 20 pA, 2 ms.