Figure 2.

Doxycycline treatment prevents matrix degradation and remodeling. Trichiasis fibroblasts F10 and F11 were embedded in collagen gels in medium with/without 416 μM doxycycline. The gels were fixed and stained with rhodamine phalloidin after 7 days. Shown are representative images of cells embedded in the matrix: red, two-dimensional projection of the full cell F-actin volume; white, collagen matrix fibers viewed using confocal reflection microscopy. Arrows show pericellular collagen fibers compaction. Arrowhead radial alignment consecutive to cell dynamic activity. Scale bar, 10 μm.