Figure 5.

Rapid loss of mAb 6E10 immunoreactivity with persistent OC antibody reactivity. A separate cohort of APPsi:tTA mice with the same B6/FVB F1 genetic background were aged out to 22 months and then given DOX diet as noted on the figure. Cryopreserved frozen sections were immunostained with mAb 6E10 or the polyclonal OC as described in Materials and Methods. A, C, E, By 5 weeks after DOX treatment, cellular reactivity with mAb 6E10 disappears (arrow), whereas amyloid plaque staining (arrowhead) persists. B, D, F, The OC antibody preferentially recognizes amyloid deposits. We noted some punctate cellular reactivity of the OC antibody in the CA layer of the hippocampus, but this staining was present in NTg mice (G) and was also seen with secondary antibody alone (H).