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. 2013 Jan 9;33(2):495–506a. doi: 10.1523/JNEUROSCI.3710-12.2013

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Copyright © 2013 the authors 0270-6474/13/330495-12$15.00/0

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Figure 3. — Differential effects of testing for cue-reinforced behavior on mGluR1/5 expression within PFC subregions during protracted withdrawal from self-administered cocaine. Immunoblotting for the change in total mGluR1 and mGluR5 protein expression within the vmPFC and dmPFC was conducted on tissue derived from saline- and cocaine-experienced animals tested for cue-reinforced behavior over a 2 h period (Cue Test) or left undisturbed in their home cages (No Cue Test) at 3 or 30 d after a 10 d history of extended access (6 h/d) to either cocaine (Coc6h) or saline (Sal6h). Sample sizes ranged from 9 to 12 per group per withdrawal time point. A, A direct comparison of receptor protein expression within the vmPFC of Sal6h and Coc6h animals across the two withdrawal time points confirmed a time-dependent reduction in mGluR1/5 expression in Coc6h animals that were tested for cue-reinforced behavior before tissue harvest. B, No statistically reliable change in vmPFC receptor expression was apparent in Coc6h rats that were not tested for cue-reinforced behavior before tissue collection. C, In contrast to the vmPFC, immunoblotting on dmPFC of these same animals revealed elevated receptor levels in Coc6h animals tested for cue-reinforced behavior at both 3 and 30 d withdrawal. D, However, as also observed for the vmPFC, the changes in dmPFC mGluR1/5 expression was not apparent in animals left undisturbed before tissue harvest. Sample sizes are indicated in parentheses. *p < 0.05 versus respective data at 3 d withdrawal.