Figure 3.

Heterotopia contained neuroblasts, astrocytes, and cells with a neuronal morphology at P19. A, B, Coimmunostaining for GFAP (green) and DCX (blue) in a sagittal section containing Rheb^CA-expressing cells at the RMS_elbow. C, Confocal image of DCX immunostaining in the Rheb^CA and control conditions. Image for the Rheb^CA condition is the same as that shown in A and B. D, E, Immunostaining for GFAP (green) and DCX (blue) of the cells shown in the white square in A and B. The top and deep sections are shown in D and E, respectively. The arrows point to RFP⁺ cells being DCX⁺ or GFAP⁺. F, Immunostaining for GFAP (green) of Rheb^CA-expressing cells in a heterotopia at the RMS_elbow. The arrows point to RFP⁺ cells being GFAP⁺. Scale bars: A–C, 150 μm; E, 30 μm; F, 10 μm.