Figure 8.

OB heterotopia, micronodules, and dendrite hypertrophy are mTOR dependent. A, B, Images taken in a medial sagittal section of the OB illustrating cells forming micronodules in the vehicle condition compared with the rapamycin condition (B) and control (GFP instead of Rheb^CA) conditions (A). In the rapamycin and vehicles, cells were electroporated with Rheb^CA. C, Bar graphs of the percentage of micronodules (or clusters) in the Rheb^CA condition with vehicle (red) or rapamycin (green) normalized to the GFP-electroporated condition (blue). D, E, Images of ectopic GFP- or Rheb^CA-containing cells in or outside the RMS_OB in sagittal sections. The rectangles highlight the analyzed regions. F, Bar graphs of the number of ectopic cells in the OB in the GFP- (blue) and Rheb^CA-electroporated conditions with vehicle (red) or rapamycin (green). G, Images of Rheb^CA-expressing cells in the vehicle and rapamycin condition. H, Sholl analysis representing the number of intersections for the basal dendrites of Rheb^CA-expressing granule cells in vehicle (red)- and rapamycin (green)-treated mice at 19 dpe. I, Immunoblots of phospho-S6^240/244 (pS6) and S6 from Neuro2a transfected with a control vector (GFP), mTOR-encoding, or Rheb^CA-encoding vector. CTL, Control. J, K, Sholl analysis of the number of intersections for the basal dendrites of GFP or shRaptor (green)-expressing and mTOR or shScramble (red)-expressing granule cells at 19 dpe. Scr, Scramble; RAPA, rapamycin. *p < 0.05, **p < 0.005, ***p < 0.001. Scale bars: A, B, 100 μm; D, E, 350 μm; G, 50 μm.