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. 2013 Jan 16;33(3):936–949. doi: 10.1523/JNEUROSCI.2749-12.2013

Figure 9.

Figure 9.

Effect of expression of cationic channels in OLQ neurons and K+ channels in socket glia on delm-1 knock-out nose-touch defects. A, Ratios of reversals (dark-gray bar) and head withdrawals (light-gray bar) assayed at 22°C for N2 (wild type, 0.640 ± 0.022 and 0.160 ± 0.017), delm-1 (0.375 ± 0.026 and 0.065 ± 0.016), delm-1 expressing mosquito TRPA1 (AgTRPA1) in OLQ neurons (delm-1(ok1226);Pocr-4::AgTRPA1, 0.430 ± 0.030 and 0.070 ± 0.019), trpa-1 knock-out (0.290 ± 0.025 and 0.045 ± 0.013), and trpa-1 knock-out expressing AgTRPA1 in OLQ neurons (trpa-1(ok999);Pocr-4::AgTRPA1, 0.265 ± 0.029 and 0.065 ± 0.016). n = 80, 40, 40, 40, and 40. B, Same as in A except that behavioral assays were conducted at 28°C. N2 (wild type, 0.600 ± 0.024 and 0.147 ± 0.017), delm-1 (0.325 ± 0.028 and 0.090 ± 0.020), delm-1 expressing mosquito TRPA1 (AgTRPA1) in OLQ neurons (delm-1(ok1226);Pocr-4::AgTRPA1, 0.640 ± 0.026 and 0.100 ± 0.017), trpa-1 knock-out (0.235 ± 0.028 and 0.050 ± 0.013) and trpa-1 knock-out expressing AgTRPA1 in OLQ neurons (trpa-1(ok999);Pocr-4::AgTRPA1, 0.235 ± 0.032 and 0.070 ± 0.018) (n = 80, 40, 40, 40, and 40). C, Example of ionic currents recorded in an oocyte injected with IRK-2 cRNA and perfused with a solution containing 100 mm KCl. Currents were elicited by voltage steps from −160 to + 80 mV in 20 mV increments. The dashed line is the zero current level. IRK-2 currents were obtained by subtracting the BaCl2 resistant current from the control currents. BaCl2 is a blocker of inward rectifier K+ channels and was used at the concentration of 1 mm. D, Average IRK-2 current–voltage relationship shows that this channel conducts outward current (n = 12). E, Ratios of reversals (dark-gray bar) and head withdrawals (light-gray bar) of N2 (wild type), delm-1, delm-1 expressing IRK-2 in socket glia, delm-2;delm-1 double mutant expressing IRK-2 in socket glia, and wild type expressing IRK-2 in socket glia (n = 160, 40, 50, and 30, respectively). F, Same as in E for N2 (wild type), trpa-1 knock-out, and trpa-1 knock-out expressing IRK-2 in socket glia (n = 40 for each). Data are means ± SE. **p < 0.01 (ANOVA); NS, nonsignificant.