Figure 2.

Temporal dynamics of dopaminergic neuron responses to cooling. A, Example of a GCaMP calcium trace recorded from MP1 during cooling, along with the temperature trace from the same recording. This neuron reached a defined response threshold (mean + 3 SDs) at 22.7°C (Δtemp = 2.3°C), marked by the intersection of the red dashed lines. B, Threshold temperature (Δtemp; mean ± SEM) calculated for each subset of dopaminergic neurons. C, Time from the onset of cooling to the peak change in temperature (temperature minimum) and the peak dopaminergic neuron response. *p < 0.05 (Bonferroni's test), **p < 0.01 (two-way ANOVA). The MV1 neurons peaked while the temperature was still falling.