Figure 5.

Dopaminergic responses to cooling after experimental manipulations. A, Responses in MP1 to brief cooling in controls (replotted from Fig. 1), animals with surgical manipulations [ablation of the antennae, antennae plus maxillary palps (Ant + MP), or legs], or addition of 1 μm TTX to the bath solution. Mean (dark lines) ± SEM (light surrounds) are shown. Vertical dashed gray lines show the time window (90–110 s) in which the mean response was calculated for B. B, Mean responses of MV1 and MP1 neurons to the experimental manipulations in A, plus experiments in which the basal temperature was set at 32°C and then briefly cooled to 25°C. Statistical significance of the pairwise comparison between the peak responses of each group with the control group is indicated by asterisks above the bars, and significance of the peak response to the prestimulation ΔF/F magnitude is indicated below the bars (Peak vs Pre). *p < 0.05, **p < 0.01, ***p < 0.001 (Bonferroni's test).