Figure 7.

The effects of silencing subsets of dopaminergic neurons on temperature-preference behavior. A, The behavioral apparatus and assay conditions. The bottom shows an illustration of the experimental setup, and the mean recorded temperatures across the gradient are graphed at the top (n = 60). B, Illustration of temperature shifts for TARGET experiments. Flies were raised at 18°C and then either kept at 18°C or transferred to 30°C for 3–4 d to induce Kir2.1 expression. C, Illustration of the dopaminergic neurons from the PPL1 cluster that innervate the mushroom body lobes. The mushroom body lobes are outlined, and each subset of dopaminergic neurons is illustrated in a different color. The legend indicates the innervation region of each GAL4 driver. The α and α′ tips are innervated by dopaminergic neurons that are not yet delineated by individual GAL4 drivers. The V1, MV1, and MP1 neurons innervate the upper stalk, lower stalk/junction, and heel, respectively. D, Dorsal; L, lateral; P, posterior. D–G, Distribution of flies across the temperature gradient, with and without induced Kir2.1 expression under control of different GAL4 drivers. The gray arrows in D indicate the bins that were summed to calculate avoidance indices against high and low temperature. H, Avoidance index against low temperatures (AI_low) for all GAL4 lines described in this study. ***p < 0.001 (Bonferroni's test). I, Avoidance index against high temperatures (AI_high) for all GAL4 lines described in this study.