Skip to main content
. 2013 Jan 30;33(5):1846–1857. doi: 10.1523/JNEUROSCI.4284-12.2013

Figure 4.

Figure 4.

Rho inhibition protects against dendrite loss in Arg knockdown neurons. A, Camera lucida drawings of dendrites from neurons transfected with vector, shArg, shArg with RhoA-N19, and transfected with shArg then treated with Y-27632. Scale bar, 100 μm. B, Examples of Arg knockdown neurons with Rho blockade via RhoA-N19 expression or Y-27632 treatment. Arg knockdown neurons with inhibition of Rho signaling still have fewer dendritic spines compared with control neurons. Scale bar, 2 μm. C–F, Dendrite branch tip number (C), total dendrite length (D), spine density (E), and filopodia ratio (F) in control- or shArg-transfected neurons. Coexpressing RhoA-N19 and Y-27632 treatment prevents dendrite arbor loss in Arg knockdown neurons. However, spine density and filopodia ratio are not affected. ***p < 0.001 when compared with control vector; #p < 0.05, ###p < 0.001 when compared with shArg, one-way ANOVA with post-Dunnett's multiple-comparison test. n = 16–30 neurons, three independent cultures.