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. Author manuscript; available in PMC: 2013 Dec 13.
Published in final edited form as: Nature. 2013 May 5;498(7453):251–254. doi: 10.1038/nature12106

Figure 1. Non-random segregation of Y and X chromosome strands during GSC divisions.

Figure 1

a) Chromosome-specific probes used in this study. b) Schematic diagram of the CO-FISH procedure. Cy3- and Cy5-labelled probes for the Y chromosome are shown as an example. Pavarotti-GFP27 (midbody/ring canal), ShAdd-Venus28 or anti-Add antibody (spectrosome) was used to identify GSC-GB pairs. Representative images of CO-FISH results using Y chromosome probes (c, d), X chromosome probes (e, f), and both X and Y probes (g–i). Expected segregation patterns based on co-segregation vs. random segregation are shown at the bottom of (g–i). In all figures the Cy5 signal is indicated by solid arrowheads and the Cy3 signal by open arrowheads. (*) Hub. N, number of GSC-GB pairs scored. Data are presented as mean ± standard deviation.