Figure 7.

Increased θ and γ frequency recruitment of DGCs during postnatal development. A, B, Responses of DGCs from P12 (black bars) and P60 (gray bars) animals to varying frequency stimulation of the PP (1, 5, 10, and 60 Hz). A, Raw mean percentage activation. B, Percent recruitment normalized to the activation evident during 1 Hz stimulation. Note that P12 DGCs, although more active overall, exhibited very little frequency recruitment, whereas P60 DGCs were significantly recruited by θ (5–10 Hz) and γ (60 Hz) frequency stimulation of the PP (two-way ANOVA). Both frequency and age effect are significant (p < 0.0001). *p < 0.05, 1 vs 10 Hz for P60 (Bonferroni post hoc test). ***p < 0.001, 1 vs 60 Hz for P60 (Bonferroni post hoc test). n = 3 animals, 6 slices, 11 fields, 629 cells for P12; n = 3 animals, 4 slices, 5 fields, 366 cells for P60.